Glial response of the cortex of two hemispheres of the brain of animals following electrical stimulation of partial and generalized epileptic seizures (experimental study)

Glia plays a significant role in epileptogenesis. As a known, glial reactions occur in the epileptogenic focus. In this study we analyzed the glial proliferation in the cortex of two hemispheres of the brain of rodents.

PURPOSE OF THE STUDY: to analyze the glial reaction in the cerebral cortex of rats which occurs by electrical stimulation of epileptic seizures.

MATERIALS AND METHODS: 82 Wistar rats were included in experimental study. The first group (n=10) — intact control; the second group (n=20) — animals with intracerebral electrode implantation, without stimulation; the third group (n=20) — animals with partial epileptic seizures; the fourth group (n=20) — animals with generalized epileptic seizures. An immunohistochemical study was performed with markers of glial cell.

RESULTS. The proliferative activity of astrocytes and microglial cells in the both cerebral cortex of rodents after stimulation of partial and generalized seizures and also oligodendrocytes of the right cortex of the brain of rodents after stimulation of generalized seizures were detected.

CONCLUSION. Electrical stimulation of partial and generalized seizures is accompanied by reactive proliferation of astrocytes and microglial cells in the cortex of the right and left hemispheres of the brain of experimental animals and oligodendrocytes in the cortex of the right hemisphere of the brain of rodents during electrical stimulation of generalized seizures.

1. Magalhаes DM , Pereira N, Rombo DM , Beltrаo-Cavacas C, Sebastiаo AM , Valente CA. Ex vivo model of epilepsy in organotypic slices — a new tool for drug screening. Journal of Neuroinflammation. 2018;15(203):18. https://doi.org/10.1186/s12974‑018‑1225‑2

2. Lukasiuk K, Becker AJ. Molecular Biomarkers of Epileptogenesis. Neurotherapeutics. 2014;11:319–323. https://doi.org/10.1007/s13311‑014‑0261‑6

3. Aleksandrov MV, Ulitin AY u, Bersnev VP, Pavlovskaya ME , Abramov KB, Arkhipova NB. Structural and functional organization of the epileptic focus. Rossiiskii neirokhirurgicheskii zhurnal imeni professora A. L . P olenova. 2017; 9(2):5–9. (In Russ.).

4. Kuralbaev AK, Zabrodskaya YuM, Ivanova NE, Kasumov VR, Bersnev VP, Telegina AA , Sitovskaya DA , Gokhman EA . Pathological changes in the hippocampus in temporal lobe epilepsy. Rossiiskii neirokhirurgicheskii zhurnal imeni professora A. L . P olenova. 2017; 9(2):5–9. (In Russ.).

5. Cordero-Arreola J, West RM , Mendoza-Torreblanca J, Mendez-Hernandez E, Salas-Pacheco J, Menendez-Gonzalez M, Freire RC, Machado S, Murillo-Rodriguez E, Nardi A, Arias-Carrion O. The Role of Innate Immune System Receptors in Epilepsy Research. CNS Neurol Disord Drug Targets. 2017;16(7):749–762. https://doi.org/10.2174/1871527316666170725145549

6. European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Explanatory Report. Strasbourg.1986;123. Accessed March 18, 1986. http://www.worldlii.org/int/other/treaties/COETSER/1986/1.html

7. Sitovskaya DA , Zabrodskaya YuM, Sokolova TV, Kuralbaev AK, Nezdrovina VG, Dobrogorskaya LN. Structural heterogeneity of epileptic foci in local pharmacoresistant epilepsy. Archiv patologii. 2020; 82(6):5–15. (In Russ.). https://doi.org/10.17116/patol2020820615

8. Sukhovaya AI, Pimonov DA , Zabrodskaya Yu M . Inflammatory reactions in epileptic foci in drug-resistant epilepsy. Izvescia Rossiiskoi voenno-medicinskoi academii. 2020; 39(1–2):170–173. (In Russ.).

9. Litovchenko AV, Zabrodskaya YuM, Sitovskaya DA , Khuzhakhmetova LK, Nezdrovina VG, Bazhanova E . D . Expression of markers of neuroinflammation and apoptosis in the temporal lobe of the brain in patients with drug-resistant epilepsy. Zhurnal iovalutionnoi biochimii i phisiologii. 2021; 57(5):411–419. (In Russ.). https://doi.org/10.31857/S004445292105003X

10. Zabrodskaya Y . M ., Gaykov, O. N., Alexandrov M . V. et al., Paramonova, N. M., Sitovskaya, D. A., Litovchenko, A. V., Sokolova, T. V., Cherenkova, S. E., & Bazhanova, E. D. (2022). Epileptic Focus in Drug-Resistant Epilepsy: Structure, Organization, and Pathophysiology. In (Ed.), Epilepsy — Seizures Without Triggers [Working Title]. IntechOpen. https://doi.org/10.5772/intechopen.108395

11. Sokolova, T. V. Zabrodskaya, Y.M.; Litovchenko, A.V. et al. Paramonova, N.M.; Kasumov, V.R.; Kravtsova, S.V.; Skiteva, E.N.; Sitovskaya, D.A.; Bazhanova, E. D . Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy. Int. J. Mol. Sci. 2022, 23, 12561. https://doi.org/10.3390/ijms232012561