Membrane-bound heat shock protein mHsp70 as a marker for malignant brain tumors
R. B. Tagaeva,
D. E. Bobkov,
A. S. Nechaeva,
E. V. Federov,
M. V. Dikonenko,
N. М. Yudintceva,
K. A. Samochernykh,
A. V. Kim,
M. A. Shevtsov https://doi.org/10.56618/2071-2693_2023_15_2_98
Abstract
Summary. The membrane-bound heat shock protein mHsp70 is selectively expressed on the surface of tumor cells, but not on normal cells; it makes mHsp70 a promising target for theranostics of malignant tumors. Objective: in vivo visualization of mHsp70-positive cells in malignant human brain tumors. Materials and methods: intraoperative sampling of histological material was carried out from 7 patients with malignant tumors and from 3 patients with epilepsy. Localization of Hsp70 was determined using live confocal microscopy. To identify intergroup differences in the intensity of green pixels (Hsp70), the Kruskal-Wallis test was applied. Results. A method for intravital staining of tumor samples and image registration by the laser scanning confocal microscopy was developed. High expression of mHsp70 was found in tumor cells compared to control cells in the brain tissue of patients with epilepsy. Conclusion. For the first time, in vivo visualization of mHsp70-positive cells in malignant brain tumors was performed. The results indicate the possibility of using mHsp70 as a marker of tumor cells.
About the Authors
R. B. Tagaeva
Institute of Cytology of the Russian Academy of Sciences (RAS), Ministry of Science and Higher Education of Russia; Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
4, Tikhoretskiy av., Saint Petersburg, 194064
2, Akkuratova str., Saint Petersburg, 197341
D. E. Bobkov
Institute of Cytology of the Russian Academy of Sciences (RAS), Ministry of Science and Higher Education of Russia; Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
4, Tikhoretskiy av., Saint Petersburg, 194064
2, Akkuratova str., Saint Petersburg, 197341
A. S. Nechaeva
Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
2, Akkuratova str., Saint Petersburg, 197341
E. V. Federov
Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
2, Akkuratova str., Saint Petersburg, 197341
M. V. Dikonenko
Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
2, Akkuratova str., Saint Petersburg, 197341
N. М. Yudintceva
Institute of Cytology of the Russian Academy of Sciences (RAS), Ministry of Science and Higher Education of Russia; Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
4, Tikhoretskiy av., Saint Petersburg, 194064
2, Akkuratova str., Saint Petersburg, 197341
K. A. Samochernykh
Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
2, Akkuratova str., Saint Petersburg, 197341
A. V. Kim
Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
2, Akkuratova str., Saint Petersburg, 197341
M. A. Shevtsov
Institute of Cytology of the Russian Academy of Sciences (RAS), Ministry of Science and Higher Education of Russia; Personalized Medicine Centre, Almazov National Medical Research Centre, Ministry of Health of Russia
Russian Federation
Russian Federation
4, Tikhoretskiy av., Saint Petersburg, 194064
2, Akkuratova str., Saint Petersburg, 197341
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Review
For citations:
Tagaeva R.B., Bobkov D.E., Nechaeva A.S., Federov E.V., Dikonenko M.V., Yudintceva N.М., Samochernykh K.A., Kim A.V., Shevtsov M.A. Membrane-bound heat shock protein mHsp70 as a marker for malignant brain tumors. Russian Neurosurgical Journal named after Professor A. L. Polenov. 2023;15(2):98-101. (In Russ.) https://doi.org/10.56618/2071-2693_2023_15_2_98
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