Structural changes of dystrophin in skeletal muscles in patients with chronic impairment of consciousness
Abstract
Оne of the significant limiting factors of rehabilitation of patients with chronic disorders of consciousness (DOC) is critically illness polyneuropathy (CIP) which leads to severe structural lesions of the striated muscles. Dystrophin protein is one of the structural membrane proteins of muscle fibers and carry the supporting, protective and trophic function of muscle fiber.
OBJECTIVE: To study changes in the dystrophin protein in skeletal muscle biopsies in patients with DOC and CIP.
MATERIALS AND METHODS: The results of an in 9 patients with chronic impaired consciousness, polyneuropathy of critical conditions are presented. The scope of the study was 28 biopsies, of which 10 were deltoid muscles, 10 were flounder muscles and 8 were calf muscles. Immunohistochemical study of the accumulation of dystrophin protein in the membrane of muscle fibers immunohistochemical reactions were performed using a primary antibody to dystrophin and a DBS imaging system according to standard protocols.
RESULTS: The results of an immunohistochemical study of the accumulation of dystrophin protein in the membrane of muscle fibers in 9 patients with chronic impaired consciousness, polyneuropathy of critical conditions are presented. A different degree of intensity of the accumulation of dystrophin protein in the sarcolemma, up to its disappearance, was noted. These changes were focal in nature.
CONCLUSION: Thus, atrophic changes in skeletal muscles in patients with polyneuropathy are accompanied by the disappearance of dystrophin from the sarcolemma, which predisposes to fatty degeneration of muscles. The results obtained can be used to develop measures aimed at restoring dystrophin and stabilizing sarcolemma.
Keywords
skeletal muscles,
dystrophin,
muscle weakness,
muscle atrophy,
chronicdisorders of consciousness,
critically illness polyneuropathy
Supporting agencies: The work was supported by RFFI grant № 19-29-01066.
About the Authors
E. N. Skiteva
“Polenov Neurosurgical Institute — branch of Almazov National Medical Research Centre”; State Research Center of the Institute of Medical-Biological Problems of the Russian Academy of Science
Russian Federation
Russian Federation
Skiteva Ekaterina Nikolaevna
Moscow
Yu. M. Zabrodskaya
“Polenov Neurosurgical Institute — branch of Almazov National Medical Research Centre”
Russian Federation
Russian Federation
ZabrodskayaYulia Mikhailovna
Moscow
L. N. Dobrogorskaya
“Polenov Neurosurgical Institute — branch of Almazov National Medical Research Centre”
Russian Federation
Russian Federation
Kondratyev Sergey Anatolievich
Moscow
S. A. Kondratyev
“Polenov Neurosurgical Institute — branch of Almazov National Medical Research Centre”
Russian Federation
Russian Federation
Dobrogorskaya Lybov Nikolaevna
Moscow
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Review
For citations:
Skiteva E.N., Zabrodskaya Yu.M., Dobrogorskaya L.N., Kondratyev S.A. Structural changes of dystrophin in skeletal muscles in patients with chronic impairment of consciousness. Russian Neurosurgical Journal named after Professor A. L. Polenov. 2022;14(2):150-152. (In Russ.)
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