Features of the formation of epidural fibrosis in the experiment
Abstract
Postoperative epidural fibrosis develops and progresses over time.
PURPOSE OF THE STUDY: to conduct spatiotemporal analysis of changes in the shape and size of the dural sac during the development of epidural fibrosis in the area of laminectomy.
MATERIALS AND METHODS. The experiment was carried out on male rats of the Wistar breed. Animals underwent laminectomy at the level of LVI–SI. On days 3, 7, 14, 21 and 28, the rats were taken out of the experiment and a histological examination was performed in the laminectomy zone with hematoxylin and eosin staining. To assess the dynamics of tissue changes, the anteroposterior and transverse dimensions of the dural sac and the ratio of the transverse to anterior-posterior were measured. The images were evaluated in the Image J program (Wayne Rasband, USA), compared with control data (intact animals), and the sizes were measured in pixels. Statistical analysis was performed using SPSS 21.0 (IBM Corp., USA) and Microsoft Office Excel 2016 (Microsoft Corp., USA).
RESULTS. It has been established that during the formation of epidural fibrosis, the shape and size of the dural sac changes. Multivariate logistic regression analysis showed a significant increase in the transverse dimension (χ2 = 0.019 at p < 0.05), while the anterior-posterior or longitudinal dimension suffered less, nevertheless, it decreased by more than 10 % of the control (χ2 = 0.023 at p < 0.05).
CONCLUSION. The experiment revealed the dynamics of the transformation of the shape and size of the dural sac in the area of formation of the connective tissue scar after laminectomy. Early involvement in the process of scar formation of the dura mater was noted.
About the Authors
A. P. Zhivotenko
Irkutsk Scientific Centre of Surgery and Traumatology
Russian Federation
Russian Federation
Irkutsk
I. A. Shurygina
Irkutsk Scientific Centre of Surgery and Traumatology
Russian Federation
Russian Federation
Irkutsk
O. A. Goldberg
Irkutsk Scientific Centre of Surgery and Traumatology
Russian Federation
Russian Federation
Irkutsk
V. A. Sorokovikov
Irkutsk Scientific Centre of Surgery and Traumatology
Russian Federation
Russian Federation
Irkutsk
S. N. Larionov
Irkutsk Scientific Centre of Surgery and Traumatology
Russian Federation
Russian Federation
Irkutsk
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Review
For citations:
Zhivotenko A.P., Shurygina I.A., Goldberg O.A., Sorokovikov V.A., Larionov S.N. Features of the formation of epidural fibrosis in the experiment. Russian Neurosurgical Journal named after Professor A. L. Polenov. 2022;14(1-2):42-45. (In Russ.)
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